Stimulation of Ca-induced Ca release only transiently increases the systolic Ca transient: measurements of Ca fluxes and sarcoplasmic reticulum Ca

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Stimulation of Ca-induced Ca release only transiently increases the systolic Ca transient: measurements of Ca fluxes and sarcoplasmic reticulum Ca.

OBJECTIVE To investigate the effects of stimulating calcium induced Ca release with low concentrations (100-200 microM) of caffeine and, in particular, to study the cellular mechanisms responsible for the transient responses found previously. METHODS Experiments were performed on isolated rat ventricular myocytes. Intracellular calcium concentration ([Ca2+]i) was measured with Indo-1, the cel...

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Sarcoplasmic Reticulum Ca Refilling Controls Recovery From Ca -Induced Ca Release Refractoriness in Heart Muscle

In cardiac muscle Ca -induced Ca release (CICR) from the sarcoplasmic reticulum (SR) is initiated by Ca influx via L-type Ca channels. At present, the mechanisms underlying termination of SR Ca release, which are required to ensure stable excitation-contraction coupling cycles, are not precisely known. However, the same mechanism leading to refractoriness of SR Ca release could also be responsi...

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Sarcoplasmic Reticulum Luminal [Ca

The role that sarcoplasmic reticulum (SR) luminal Ca 2+ concentration plays in regulating ryanodine receptors (RyRs) is considered to be very important in process of cardiac excitation-contraction coupling. Here we developed a multi-scale mathematical model by coupling a two-dimensional spatio-temporal Ca 2+ reaction-diffusion model with an action potential model of the ventricular myocyte. The...

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Biphasic decay of the Ca transient results from increased sarcoplasmic reticulum Ca leak

KEY POINTS Ca leak from the sarcoplasmic reticulum through the ryanodine receptor (RyR) reduces the amplitude of the Ca transient and slows its rate of decay. In the presence of β-adrenergic stimulation, RyR-mediated Ca leak produces a biphasic decay of the Ca transient with a fast early phase and a slow late phase. Two forms of Ca leak have been studied, Ca-sensitising (induced by caffeine) an...

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Effects of ischemia on sarcoplasmic reticulum Ca uptake and Ca release in rat skeletal muscle

Tupling, R., H. Green, G. Senisterra, J. Lepock, and N. McKee. Effects of ischemia on sarcoplasmic reticulum Ca21 uptake and Ca21 release in rat skeletal muscle. Am J Physiol Endocrinol Metab 281: E224–E232, 2001.—In this study, we investigated the hypothesis that prolonged ischemia would impair both sarcoplasmic reticulum (SR) Ca21 uptake and Ca21 release in skeletal muscle. To induce total is...

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ژورنال

عنوان ژورنال: Cardiovascular Research

سال: 1998

ISSN: 0008-6363

DOI: 10.1016/s0008-6363(97)00266-6